hi.. a question from a very new user here.. I have extracted the 16S rRNA from stool samples and performed Sanger sequencing to ID the bacteria population present in the samples (I have both forward and reverse sequences for each one.). Therefore, I had ab1 files.
i have converted the AB1 files to fastq files using a online software..
Now I am facing trouble to import my data to Qiime.
i searched about this topic and found similar question at Profile - diegorg - QIIME 2 Forum. however according the forum session, the author was suggested to convert the ab1 files to fastq using tracy..
- i downloaded tracy, but i dont know how to convert ab1 files to fastq.
- i tried to import data using my previously converted file to Qiime by following the suggestion in the forum but unsuccessful.
any suggestions please?