Importing FASTQ.GZ

[king20]

This is my first time to use QIIME2. I followed the tutorial on Importing demultiplexed sequence data:

Using the Upload Data tool:

• Steps to setup data_to_import:sequences:
  1. On the fourth tab (Rule-based):
  2. Set “Upload data as” to Collection(s)
  3. Set “Load tabular data from” to Pasted Table
  4. Paste the following contents into the large text area:

In the tutorial, there are 2 columns, I think it is file name and URL.

FMT.0093C_46_L001_R2_001.fastq.gz https://qiime2-workshops.s3.us-west-2.amazonaws.com/faes-jan2022/data/020-tutorial-upstream/030-importing/data_to_import/FMT.0093C_46_L001_R2_001.fastq.gz

My question is where can I get the URL of my sequences. I only have the fastq.gz files of my sequences. I dunno where to find or create it.

I'm a beginner so please bare with me.

[Oddant1]

Hello @king20, what type of data are you trying to upload? Is it SampleData[PairedEndSequencesWithQuality] like the tutorial you are referencing, or is it something else?

[king20]

It is fastq.gz files fresh from the sequencing facility.

[Oddant1]

Sorry, I should have phrased my question differently. So we know the data has quality, but we need a bit more information to know how to import it into QIIME 2. Is it paired end or single end data? Is it in casava one eight format? Once I have all the information needed to import this data into QIIME 2, I'll provide step by step instructions on uploading the data to galaxy and importing it into a QIIME 2 artifact.