Hello,
First, thank you for this amazing community.
I received a ZIP archive of sequence(FASTA) files for each sample after pre-processing ,i.e. read merging, adapter&quality trimming, and chimeric checking.
I want to import these files to qiime2 and perform an analysis with Deblur and feature table construction.
Can you please help me understand how this is done? I couldn't find a solution in the import manual.
Thank you,
Lena
Have you tried converting them to fastq?
May be this will help
https://docs.qiime2.org/2020.6/tutorials/importing/#sequences-without-quality-information-i-e-fasta
Hi @LenaLapidot,
I think @steffi has provided wonderful advice, but I want to re-iterate @Angelica's question, because deblur needs sequences with quality in QIIME 2. (edit: The amazing @Nicholas_Bokulich pointed out that you can denoise using just vanilla deblur without quality scores.)
You could also still do OTU clustering in QIIME.
Best,
Justine
Unfortunately I don't have the quality scores..
Thank you, that was the plan, however I have multiple FASTA files - each sample has a separate FASTA file, so I have to covert them all to sequences.fna first..
Do you have a suggestion ?
Thank you 
Perfect! Thank you for this important advise
How can I do the vanilla Deblur without the quality scores?
Hi @LenaLapidot,
I haven't done it recently, but I think if you run the command deblur on your terminal, you'll get help documentation. You could. also check the github page.
Best,
Justine
Hi @LenaLapidot
Yeah. I think you to convert your fastq files into sequence.fna and then upload. U can achieve this using qiime1. Preparing raw Illumina data in different formats for use with QIIME — Homepage.
As far as I know, We can do this in qiime2 pipeline. Correct me if I am wrong
Updating that I've installed deblur (conda) and the Deblur command worked Now I am trying to continue with qiime2 analysis, mainly for the tree and taxonomy. If there's anything I should pay attention to, please let me know. Thank you for the help and the support!
Thank you, Steffi! I've done this with the split libraries command. However, it looks that I have to continue with OTU clustering.. so now I'm trying to go with the "vanilla" Deblur option.
Hi,
I am not familiar with vanila Deblur option. Its good that you found a way to go ahead.
Hi @LenaLapidot,
At thsi point, I'd go forward with your favorite tree building and taxonomy assignmeent algorithm. I think the tutorials give a nice set of options.
Best,
Jusitne
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