but I get an error code stating that it is not in DNAFASTA format file. is that because it is a text file formatted in one line with no line breaks? If I add line breaks can I import using this format?
Thanks so much for help! I was able to upload the data after I fixed the format of the fasta file. Sorry to bombard you with questions, but I am now trying to demultiplex the data now with the following command:
Could you elaborate on the purpose of this FASTA file? There's two notions of it in QIIME 2, as SampleData[Sequences] and FeatureData[Sequence]. You've imported as the latter which means you've already selected your "features" and are associating some sequence information with them. Demultiplexing doesn't really make sense in that context.
But if these are sequences for you samples, then you are probably dealing with post-split-libraries output? In which case you need to import as SampleData[Sequences] instead and use qiime vsearch to perform OTU clustering/feature-selection.