Currently I'm trying to import sequence data into Qiime2 2019.4 via Virtual Machine. I'm already processed the fastq file by removing barcodes and linkers. When I go to import the sequences into Qiime2, it gives me an error:
There was a problem importing /home/qiime2/Documents/pacbio/qiime2/emp-single-end-sequences/:
- /home/qiime2/Documents/pacbio/qiime2/emp-single-end-sequences/sequences.fastq.gz is not a(n) FastqGzFormat file:*
- Quality score length doesn't match sequence length for record beginning on line 5*
I ran the normal import command with type as EMPSingleEndSequences with no option to run "--verbose" flag.
qiime tools import --input-path '/home/qiime2/Documents/pacbio/qiime2/emp-single-end-sequences/' --type EMPSingleEndSequences --output-path '/home/qiime2/Documents/pacbio/Qiimedocs/empsingleend.qza'
Any and all help is appreciated!!