Hello Dear All While I am new to Qiime 2, infact a beginner. I have fasta file data sets with three samples, the data is already demultiplexed. Please let me know how to import this data and how to combine these sequenes ( while in qiime 1 we were having a command add_qiime_labels). how to do i…

Hi @divyaprince321 , [image] divyaprince321: while in qiime 1 we were having a command add_qiime_labels There is no equivalent action in QIIME 2. Based on your description of the data, it sounds like some pre-processing (including demultiplexing) has already been done and the data are now in …

Thank for your Concerns Does it mean that in QIIME 2 only fastq files are processed for downstream analysis

[image] divyaprince321: Does it mean that in QIIME 2 only fastq files are processed for downstream analysis Not at all. But the denoising methods used in QIIME 2 do require fastq. You can import other data types, e.g., a biom table, but fasta formats are only used for sequences post-denoising…

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