Importing Data Problem

[doudou2047 (孟琼)]

Hi, I started at pairends fastq files, so I choose "Casava 1.8 paired-end demultiplexed fastq" to import data.

However, I got the error message Missing one or more files for CasavaOneEightSingleLanePerSampleDirFmt: '.+_.+_L[0-9][0-9][0-9]_R[12]_001\\.fastq\\.gz'

The code is :
The file in the S.5981.V6_paired is:
Could you help me please?
Qiong

[doudou2047 (孟琼)]

Hi, I got the files from sequencing center. However each sample have four files:
such as: 1856
S.1856.V4_R1_tc.fastq
S.1856.V4_R2_tc.fastq
T.1856.V4_R1_tc.fastq
T.1856.V4_R2_tc.fastq
And I have no idear about the barcode.
Could you give me some suggestions?
Thank you

[thermokarst]

@doudou2047

S.1856.V4_R1_tc.fastq
S.1856.V4_R2_tc.fastq
T.1856.V4_R1_tc.fastq
T.1856.V4_R2_tc.fastq

I suspect that two of the files are the reads, and the other two are the barcodes. Can you please provide the first few lines of four files from the same sample?

[doudou2047 (孟琼)]

Thank you so much. Could you help me with that^_^
I am stuck at the Demultiplexed, due to I don't know I should extract the barcode sequence or not?
Thank you. Looking forward for your reply.

[doudou2047 (孟琼)]

Hi,
According to the size, I think it is not the barcord sequence, right?

First of all, I got qza file through this step.
Right now I am cufusing about the 4 files for one sample, and according to the “Atacama soil microbiome” tutorial, Could I check the detail from you? I need extract the barcode into the file to the metadata file, However I checked the metadata there are not including barcode sequence only in mapping file for qiime. right?
And How to deal with Barcode Sequence.

Thank you so much, I asked so many simple questions but for me is a big bug I could not eat it^
Thank you.

[thermokarst]

Thanks for the info @doudou2047.

Please ask your sequencing center for more details regarding the contents of these 4 files for each sample - we cannot know what they did or how they prepared them.

Once you here back from them about which files to use, please use the fastq manifest format to import your reads.