importing data - phared score

[karina_F]

Hi!

I’m attempting to import my data that was sequenced using Illumina Miseq. I’m using the Paired End Demultiplexed option. The program reads my manifest just fine, but I get this error:

An unexpected error has occurred:

  Decoded Phred score is out of range [0, 62].

See above for debug info.

Does anyone know of a way to figure this out so that I may continue?

Thanks!

[colinbrislawn (Colin J Brislawn)]

Hello Karina,

Welcome to the forums! :qiime2:

@karina_F

Decoded Phred score is out of range [0, 62].

Looks like this uses the PHRED 64 offset. I'm not sure this is supported by Casava 1.8 paired-end demultiplexed fastq, but it is supported by the Fastq manifest format. You can try using
--input-format PairedEndFastqManifestPhred64V2 and see if that resolves the issue with the q-score range.

Let us know if you have any questions.