Hello everyone! I´m trying to get my already demultiplexed PE sequences imported to qiime2, using the manifest protocol and coming across some issues! If anyone could help me I appreciate!!
The error I am getting back is that the file manifest.txt (or .tsv) is not a PairedEndFastqManifestPhredd33V2 file because the first column (sample id) is not recognized despite bein specified in the input file:
This is the manifest file I´ve created:
and this is the error I´m getting back:
I appreciate any suggestions! have a nice day!