Hello,
I have gone through every forum that have already had similar issues, but nothing has worked. I believe my issue stems from the file path being wrong somewhere. I am using qiime2-2022.11 through Ubuntu on a Windows PC through Linux.
When I run qiime tools import
--type 'SampleData[SequencesWithQuality]'
--input-path FINAL_Manifest_File.txt
--output-path single-end-demux.qza
--input-format SingleEndFastqManifestPhred33V2
I receive these errors:
FINAL_Manifest_File.txt is not a(n) SingleEndFastqManifestPhred33V2 file:
Filepath on line 1 and column "absolute-filepath" could not be found (/Rats/QIIME2/Rats/Fastq/1EurasianBeaver.R1.RUN1.L1.fastq.gz) for sample "1EurasianBeaver.R1.RUN1.L1".
This is my manifest file:
In my file path for Rats/QIMME2/Rats when I run "ls" too see what is in it it shows my manifest file:
Does anyone have any ideas what is going wrong?