Importing AVITI sequence data into qiime

Technical Support
1

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Hi,

I’m trying to import AVITI sequence data into QIIME, but I’m having trouble with the manifest file. I keep getting the following error: manifest.tsv is not a(n) PairedEndFastqManifestPhred33V2 file.

Could someone please share the code or guidance for creating the correct manifest file?

Thank you,
Asmaa

2

Hi @asmaamorsi,
Could you share the command that you're running and the manifest file that you're providing? A directory listing of the directory containing the files you're trying to import will also be helpful (e.g., if your .fastq.gz files are in a folder called my-data, run ls my-data and provide the output). We should be able to help you sort it out with that information.

3

Hi, Thanks for your response. I figured out the problem in the manifest file and fixed it.