Import paired, demultiplexed sequences to run through DADA2 (not casava format)

[ebolyen (Evan Bolyen)]

Hi @Lorinda!

Sorry I didn't get back to you earlier, but great job solving the problem!

@Lorinda

I do have the unfiltered barcode file and have written code to extract only those barcodes that match the sequence files, but am unsure exactly how to proceed, or if I can for that matter… Any advice would be greatly appreciated.

@Lorinda

Problem solved! I was able to filter my barcode file to contain only those barcodes that matched my sequence file, sort both barcodes and sequences so that they were in the same order, then import into QIIME2

That is perfect, I'm glad you still had the original data somewhere!

P.S. We've got some new sequence-similarity filtering (in q2-quality-control) and taxonomy filtering (in q2-taxa) methods in this latest release, which might be useful for this dataset!