Hello everyone, I have fastq file (Ion torrent PGM platform - 400bp) that are multiplexed. I am struggling in importing it to qiime2. How can I do it? please Thanks

Hi @shwane , Many other forum users have asked this same question — have you had a chance to review those posts and try out the solutions described there? In short, I think you probably want one of the manifest formats Please look over those posts, try those solutions, and let us know specifically…

Hi @Nicholas_Bokulich thank you very much for answering my questions. My problem exactly like @ Ion torrent sequences analysis . I have one fastq file (24 samples) and I don’t have a separate barcode fastq.gz file but I can create metadata contain barcode for each sample.

[image] shwane: I have one fastq file (24 samples) and I don’t have a separate barcode fastq.gz file You could follow this tutorial to see if that works for you. [image] shwane: but I can create metadata contain barcode for each sample. So that's another option — create a separate bar…

Hey Shwane, Did you manage to run ion torrent fastq file in QIIME 2. Would it be possible to share with us how you did at least initial part where you import the data? Thanks a lot.

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