I’m starting to use qiime 2 with iontorrent and it’s not working for me !! I have only one fastq file containing 20 samples with 20 different barcodes. I also have the mappingfile that contains the sequences of barcodes and primers.
My first step was to import the data:
$ qiime tools import --input-path …/fastq/ --output-path RAW_DATA_CHIP_A.qza --type MultiplexedSingleEndBarcodeInSequence --input-format MultiplexedSingleEndBarcodeInSequenceDirFmt
But I have the following error:
There was a problem importing …/fastq/:
Missing one or more files for MultiplexedSingleEndBarcodeInSequenceDirFmt: 'forward.fastq.gz’
In the folder fastq / are the sequences: RAW_DATA_CHIP_A.fastq.gz
How could I solve that error?