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1

Hi all,
I’m starting to use qiime 2 with iontorrent and it’s not working for me !! I have only one fastq file containing 20 samples with 20 different barcodes. I also have the mappingfile that contains the sequences of barcodes and primers.
My first step was to import the data:

$ qiime tools import --input-path …/fastq/ --output-path RAW_DATA_CHIP_A.qza --type MultiplexedSingleEndBarcodeInSequence --input-format MultiplexedSingleEndBarcodeInSequenceDirFmt

But I have the following error:
There was a problem importing …/fastq/:

Missing one or more files for MultiplexedSingleEndBarcodeInSequenceDirFmt: 'forward.fastq.gz’
In the folder fastq / are the sequences: RAW_DATA_CHIP_A.fastq.gz

How could I solve that error?
Many thanks!!

2

Hey there @PatoUru!

Update this:

--input-path .../fastq

To this:

 --input-path …/fastq/RAW_DATA_CHIP_A.fastq.gz

Then you should be good to go.

3

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