My metadata file (which has been verified by keemei) looks like this: metadata.tsv (929 Bytes)
The command is successful, but when I view it in QIIME2 view online, under the drop down menu "Sample Metadata Column", it doesn't give the option to select and view it by sample ID. I can get around it by adding a duplicate column of the ID names (with a different header) though this seems redundant to have to add in the same info.
This is the expected behavior. The alpha-rarefaction visualizer works by generating multiple rarefied tables at each sampling depth between 1 and the --p-max-depth and then returning an average diversity value for all of the tables generated for a particular depth of rarefaction.
The plot produced relies on having multiple samples per group, which would not be the case if it tried to plot individual samples. You might be interested in checking out the Parkinson’s Mouse Tutorial or this video on our channel for a bit more of an in-depth discussion of the topic.
Thanks for your reply. I'm still a little confused so I think I may have not explained my issue clearly in my first post though.
I've attached a screenshot of my issue on QIIME2 view with some notes added to it.
