How do I compare known sequences to those run through dada2 or deblur?
Hi @Barbara_Campbell,
Welcome to the forum!
There are several options. I would recommend looking through our tutorials in our user documentation.
You could use one of the tutorials as a template for your analysis and compare the outputs using the known sequences to the outputs after running through dada2 and deblur.
NCBI BLAST tool could be useful if you want to compare the ASVs generated from dada2/deblur against the database.
If you need help during your analysis feel free to post here again, we are happy to help. I also recommend searching the forum for useful bread crumbs left by previous users, this is often a fastest way to get answers.
--Hannah