I have done improting demu data, using the following:
qiime tools import --type ‘SampleData[PairedEndSequencesWithQuality]’ --input-path metadata.csv
qiime demux summarize --i-data import_results.qza --o-visualization demux.qzv
qiime dada2 denoise-single
qiime quality-filter q-score
so , netxt what I should do?
I was pareparing to do the next:
qiime feature-table summarize
but I don’t have sample-metadata.tsv.
The next steps really depend on what your scientific questions are, here you can find a few tutorials that might help you decide which commands/analyses to run next.
sample-metadata.tsv is basically the main information about the samples you are processing. This is something that is normally collected when the samples are collected and without it, it’s impossible to make any sense of the sequences. As the QIIME tutorial puts it:
The mapping file is generated by the user. This file contains all of the information about the samples necessary to perform the data analysis. Suggest either generating this file or contacting the people that collected the samples.
Hope this helps.
Thanks, I have done as you suggested.
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