Hi @ptalebic,
The issue seems to be in the delimiters of your manifest file. Looks like they were made as a comma-separated values file (.csv) thus the commas appearing in your table, but you need your manifest file to be in tab-separated values format. Retry remaking your manifest file as a true .tsv and then you should be good to go.
--type 'SampleData[PairedEndSequencesWithQuality]'
--input-path manifest.txt
--output-path paired-end-demux.qza
--input-format PairedEndFastqManifestPhred64V2
There was a problem importing manifest.txt:
manifest.txt is not a(n) PairedEndFastqManifestPhred64V2 file:
Filepath on line 1 and column "forward-absolute-filepath" could not be found (/home/qiime2/alison/16sData/sequences/1_1.fq.gz) for sample "1".
I have uploaded my manifest file here. All of my sequence data is the specified path so I am unsure why it could not be found.
Hi @Mehrbod_Estaki,
I figured it out! Thank you!
I remade the manifest in google sheets and downloaded it as a .tsv.
But another problem was that my directory was bad.
I changed the path to include $PWD instead of the whole directory string that I was using before.
What a relief! Now I just have to figure out what to do next with my output file