How to train the classifier with multiple mixed forward primers?

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Hi Mike,

Thanks for you reply! Learned a lot! Wii try!

I guess the last one also applies to qiime rescript get-silva-data, like this:

## get-silva-data
qiime rescript get-silva-data \
    --p-version '138.1' \
    --p-target 'SSURef_NR99' \
    --p-include-species-labels \
    --o-silva-sequences silva-138-99-seqs.qza \
    --o-silva-taxonomy silva-138-99-tax.qza


## Dereplicate 
qiime rescript dereplicate \
    --i-sequences silva-138-99-seqs.qza \
    --i-taxa silva-138-99-tax.qza \
    --p-mode 'uniq' \
    --p-threads 8 \
    --o-dereplicated-sequences silva-138-99-seqs-derep.qza \
    --o-dereplicated-taxa silva-138-99-tax-derep.qza

##  extract-reads
qiime feature-classifier extract-reads \
   --i-sequences silva-138-99-seqs-derep.qza \
   --p-f-primer GAGTTTGATYMTGGCTCAG  \ #28F-YM
   --p-r-primer GCTGCCTCCCGTAGGAGT \ #388R
   --p-n-jobs 8 \
   --o-reads ilva-138-99-seqs-segments.qza

Many thanks!
Birong

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