How to prepare the barcodes.fastq.file

Welcome to the forum @angelrica_kuan!

I recommend discussing with the NGS company to figure out where your barcodes are located. I suspect they may be in-line in the forward and reverse sequences, in which case you can follow this tutorial to import and demux:

And note that qiime cutadapt demux-paired now supports dual-index barcodes (see the --help documentation for details).

Good luck!