How to prepare the barcodes.fastq.file

Hi, I am new user. I obtained three files from NGS company as below:

  1. MS18201-pool_R1_001.fastq.gz\
  2. MS18201-pool_R2_001.fastq.gz\
  3. Dual barcode sequences which are in excel file

My question is how to create a barcode. fastq. file?

Thanks for replying.


Welcome to the forum @angelrica_kuan!

I recommend discussing with the NGS company to figure out where your barcodes are located. I suspect they may be in-line in the forward and reverse sequences, in which case you can follow this tutorial to import and demux:

And note that qiime cutadapt demux-paired now supports dual-index barcodes (see the --help documentation for details).

Good luck!

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