I have 2 paired end file that are already demultiplexed, and i tried to import it using fastq manifest format, but i am always failed to do that, is my manifest format are wrong? iam already following the tutorial and using Phred33 and Phred64 input format. Iam really appreciate your help and suggestion
This is my manifest file
and this is my error report
Hey there @Aditya_Ragil_Suharto!
Have you had a closer look at the error message you provided? It only found 2 cells (columns) on line 2, but it was hoping to find 3. Now look at what it found for the value in the second column:
$PWD/Dompu/forward.fastq.gz.forward. Each column is created by comma-separating the values --- according to the error (and the manifest you posted), you used a period (
forward, instead of a comma (
Hope that helps!
Thank you very much Mr. @thermokarst i didn’t see that… now i already generated the demux file
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