I'm new to QIIME and am trying to use it to analyze my 16s rRNA sequencing data. I tried the general instructions laid out for importing data onto qiime but it's giving me an error. So I read on the forum that I could create a manifest file. The problem is I don't know how to create a manifest file. I'd really appreciate it if someone could please help me, on how to create a manifest file. I have my data in fastq format. I have 3 files in total, one with barcodes, and 2 for the forward and reverse sequences. I've tried both EMPpaired end sequence protocol, and the mutiplexed code as well but nothing seems to be working.
We have a guide over here:
For some reason, our tutorials often do not show up on Google. You can find a full list of them over here: Tutorials — QIIME 2 2021.8.0 documentation
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