How to convert files into .qza for qiime analysis? and how to prepare/find metadata/ mapping file?

I prepared manifest file using all FASTQ sequences and now I need to prepare a metadata file. Is it a must? Also, how to convert the manifest file (CSV) into .qza.
My facility gave me a yml file with only one line as a metadata file but I am sure this is not metadata file. metadata should be a table.

Hi @HebaHussein-1981,

This is described in the data importing tutorial.

The metadata file that is discussed here is your clinical/environmental information associated with your samples. You should have collected this (ideally) when you collected yoru samples. It might come from your animal facility, chart review, a survey, etc, etc, etc. You can see the QIIME-specific metadata requirements here.



This topic was automatically closed 31 days after the last reply. New replies are no longer allowed.