I prepared manifest file using all FASTQ sequences and now I need to prepare a metadata file. Is it a must? Also, how to convert the manifest file (CSV) into .qza.
My facility gave me a yml file with only one line as a metadata file but I am sure this is not metadata file. metadata should be a table.
This is described in the data importing tutorial.
The metadata file that is discussed here is your clinical/environmental information associated with your samples. You should have collected this (ideally) when you collected yoru samples. It might come from your animal facility, chart review, a survey, etc, etc, etc. You can see the QIIME-specific metadata requirements here.
Best,
Justine
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