How to build an OTU table using ONT sequencing

[gregcaporaso]

Hi @ana_btt, Have you reviewed this post yet:

PRONAME: Enhancing Taxonomic Accuracy with Nanopore Long-Read Metabarcoding and QIIME2

Hi everyone! Here is a post for those of you interested in combining the strengths of both QIIME2 and Nanopore long-read metabarcoding . As you may know, the sequencing technology developed by Oxford Nanopore Technologies (ONT) enables the generation of (very) long reads, which can significantly enhance the taxonomic resolution of metabarcoding approaches . For example, the entire 16S-ITS-23S operon region can be sequenced in one go. However, Nanopore sequencing has hi…

That might help you get started. Additionally there are some ONT-focused plugins on the horizon, but nothing in the distros or on the QIIME 2 Library yet.