I have to perform ANOVA test on my samples. I would like to know to analyse in qiime2. What are the commands and and how one can choose value for --p-formula.

Please see following Docstring in qiim2 tutorial.

Usage: qiime longitudinal anova [OPTIONS]

Perform an ANOVA test on any factors present in a metadata file and/or

metadata-transformable artifacts. This is followed by pairwise t-tests to

examine pairwise differences between categorical sample groups.

Parameters:

–m-metadata-file METADATA…

(multiple Sample metadata containing formula terms.

arguments will

be merged) [required]

–p-formula TEXT R-style formula specifying the model. All terms must

be present in the sample metadata or

metadata-transformable artifacts and can be continuous

or categorical metadata columns. Formulae will be in

the format “a ~ b + c”, where “a” is the metric

(dependent variable) and “b” and “c” are independent

covariates. Use “+” to add a variable; “+ a:b” to add

an interaction between variables a and b; “*” to

include a variable and all interactions; and “-” to

subtract a particular term (e.g., an interaction term).

See

https://patsy.readthedocs.io/en/latest/formulas.html

for full documentation of valid formula operators.

Always enclose formulae in quotes to avoid unpleasant

surprises. [required]

–p-sstype TEXT Choices(‘I’, ‘II’, ‘III’)

Type of sum of squares calculation to perform (I, II,

or III). [default: ‘II’]

Outputs:

–o-visualization VISUALIZATION

[required]

How to construct above formula using R-style formula specifying the model. .

Please help.