My computer has a icore 7 processor, and an 8GB RAM, even though I try to run the DADA2 program with the following command: $ qiime dada2 denoise-paired --i-demultiplexed-seqs paired-end-demux.qza --o-table table --o-representative-sequences rep-seqs --p-trim-left-f 0 --p-trim-left-r 0 …

Hi @Nasute , Can you clarify what you mean by "It locks"? Does the computer freeze or do you just mean the operation seems to take a long time. It's hard to answer exactly how long DADA2 takes or how much RAM it needs but from experience, 8Gig should be enough for most general projects, unless you'r…

Ho @Mehrbod_Estaki Thank you very much for your answer Responding to your previous message Can you clarify what you mean by "It locks"? Basically the machine goes into suspension with the screen on, that is, I can not execute any other activity while the computer is running the DADA2 program Are…

Hi @Nasute , That's actually a fairly small dataset you are trying to denoise so it shouldn't take too long... of course that's dependent on your processor, how many cores you have available on your system etc. What you are describing as 'locked up' is actually normal. As long as the last operatio…