Help with DADA2-quality filtering

Agree with @Nicholas_Bokulich on the key questions: Are your primers on your reads? And what is the length of the region you are amplifying?

This is because the cause is probably:

(1) Unremoved primers on reads -> spurious chimera detection -> few reads making it through pipeline.

OR

(2) Truncated reads not long enough to overlap in middle -> failed merging -> few reads…

See also the discussion here: Lost of data with dada2

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