Help with ANCOM result interpretation - "Reject null hypothesis" is true for all features

That is an indication that the threshold is not behaving as expected. If W is 0, then that should not be detected to be significantly different. I would aim to only investigate the species with high W scores (and definitely not those that are zero).

Btw, I gave an explanation of how the W statistic works here. Hopefully that clarifies how this statistic works!

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