Hello,Im having a problem in importing my sequences


I would really appreciate if someone could help me with this issue

Hello and welcome to the forum!

As you can read from the error message, the plugin is complaining that instead of the absolute path you provided relative path.

What is the difference?
Let's assume that you are in directory named "Example". In that directory, you have another directory "Raw" with raw reads that you want to import.
Relative path: "Raw/raw_read_1.fq.gz"
Absolute path: "/home/your_user_name/Example/Raw/raw_read_1.fq.gz"

You can run the following command to learn the path to your current directory:

!pwd

Best,

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Hello timanix,
Thank you for helping me with this previously. I rectified that error; however, now I'm facing this issue. Please do help me out.
Also, it shows that my input format is not PairedEndFastqManifestPhred33V2 or PairedEndFastqManifestPhred64V2(I've tried both). How do I go about that for my manifest files?

Hello again!
This error usually means that at least one of your fastq.gz files is corrupted. For example, connection was interrupted while downloading and some of the files are incomplete. Check all the sizes or redownload all the files and check if it will help.

Best

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Hi [timanix]
I did find an error while uploading one of the the files.
Thank you.

Regards.

1 Like