Having problems with FASTA file

User Support
1

Hi there :slight_smile:

I'm currently learning how to use Qiime 2, and i'm having a problem with the format of my file, also did the tutorials but always where with a .fastq file, i don't really know what kind of file its what i have, and where to begin with the analisys, im sorry if the question its too basic but i really spent a lot of time and still have no clue.

My Fasta File have this format:

>Sample_ID orig_bc=CGGGCTCCTTTG new_bc=CGGGCTCCTTTG bc_diffs=0
AGTAGTAGTAGTAGTAGTATAGGATTTAGGATTTA.........
>Sample_ID orig_bc=CGGGCTCCTTTG new_bc=CGGGCTCCTTTG bc_diffs=0
AGTAGTAGTAGTAGTAGTATAGGATTTAGGATTTA.........
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If anyone can help me i'll be very greatful
Thanks

3

Hi @Cristofer_Moya,

Sorry for the delayed response.

It looks like your data is QIIME 1 post-split-libraries output, which we can import and analyze, but as a question, do you still have the raw sequence data? If possible we should start with that.

Assuming you don't still have the raw data, you can follow this tutorial to do OTU picking (DADA2 isn't an option without the quality scores, and we haven't wired Deblur up to use post-split format just yet).

5

Hi @ebolyen
thank you so much :smile: , it worked really well