Hi @jwdebelius, @elsamdea and @nandreani,
I am having problems in the reconstruction of the phylogenetic tree just in this step.
I used the SILVA 128 database to complete the Sidle tutorial with my data. The database was initially downloaded following next command and processed following the tutorial.
qiime rescript get-silva-data \
--p-version '128' \
--p-target 'SSURef_NR99' \
--p-include-species-labels \
--o-silva-sequences ./silva_128_ssu_nr99_rna_seqs.qza \
--o-silva-taxonomy ./silva_128_ssu_nr99_tax.qza
When I wanted to reconstruct the Phylogenetic Tree using qiime sidle reconstruct-fragment-rep-seqs
, I dowloaded the file Silva_128_release.tgz and I used the fasta file 99_otus_aligned.fasta inside rep_set_aligned folder in the --i-aligned-sequences
parameter. I obtained the error attached in the document. It looks like I was using information from different SILVA versions but, I think that this is not the case.
Maybe I'm not using the correct aligned-sequences file?
Regards,
Andrés
qiime2-q2cli-err-iyyulykt.txt (104.5 KB)