I've run my sequences through the Qiime2 pipeline and have reached the point of calculating alpha and beta diversity metrics for my data. However, when I try and run beta diversity I get the following error: Plugin error from diversity:
Ordinations with less than two dimensions are not supported.
Debug info has been saved to /tmp/qiime2-q2cli-err-l2dzwpsf.log
This error resulted from the following code: "qiime diversity core-metrics-phylogenetic --i-phylogeny rooted-tree.qza --i-table table.qza --p-sampling-depth 4868 --m-metadata-file Plate001_Qiime.tsv --output-dir core-metrics-results"
I'm assuming that the issue is arising from an insufficient mapping file? I'm currently using just my manifest file, which I show below:
Could I fix the problem and calculate my diversity matrices by adding columns for other variables (year, sampling depth, etc?) Or are there other issues leading to this error?
Thanks for your help! I'm hoping that I'm close to running my first dataset through Qiime2.