Form of OTU (ASV)

[Nicholas_Bokulich]

@fblues

What is the meaning of the integer numbers?
(I am sorry if this is not about QIIME2.)

Denoising methods use the md5sum of the sequence as the feature ID of that sequence. This makes it possible to merge different studies that use the same exact processing parameters.

de novo OTU clustering creates an arbitrary ID for each feature. Hence, there is no way to compare these features between studies or even multiple OTU clustering runs.

It is not impossible to compare these sequences, but it is somewhat abnormal — follow this solution:

Feature-table merge and merge-seqs does not eliminate duplicate sequences

Hi @Byron_C_Crump, The "easy" way to do this would be to go back and re-process your own data with deblur, but add the --p-no-hashed-feature-ids parameter, which will cause feature IDs to be unhashed (i.e., the sequence will become its own feature ID and match the QIITA data you have). But I will offer another solution that I hope will save some time. Deblur has caused you enough headaches so I slapped this together in hope that it helps! There are more elegant ways to do this, but a bash one-…

I can't really provide support if this fails because you are venturing into unknown territory. I still suspect the OTUs and ASVs will not be comparable since you probably processed with different parameters. It would be more straightforward and feasible to import the qiime1 OTU table into QIIME 2 and collapse both tables on taxonomy.

Good luck!