lizgehret
January 6, 2026, 9:07am
4
Hi @CamilleZhao ,
The short answer is technically yes, but it also depends on what exactly you're trying to accomplish- here are a couple of good threads from other folks who were working with full-length Nanopore 16S data and what they did in QIIME 2 vs. other tools:
Hello, I'm new here and had a few questions. So the lab I'm working in doesn't have a bioinformatician, so I've taken that role and have been learning on the fly. So I did the sequencing using the 16S Barcoding Kit 24 V14 (SQK-16S114.24) protocol and the MinION MK1C sequencing device. I selected the super high accuracy base caller, demultiplexed it, and trimmed the adapter/primers using guppy (v6.3.8). After that, I filtered based on length and quality using chopper. Everything up to here was fi…
Hi everybody, I am new here and I have no better idea than to do something crazy. I have no idea if it is possible due to the fact of the sequencing nature. But could I map ASV reference sequences produced by QIIME2 into 16S full-length nanopore sequences?
Both the Illumina (MiSeq 2 × 250 paired-end) and nanopore sequencing were made with soil samples.
My final goal is to produce an ASV ID abundance table with the nanopore data. So as to "compare" the samples sequenced with Illumina with the n…
Hope this helps!
Cheers
