I want to use picrust2 in qiime2. But it requires .fna file. So i cant understand how to generate .fna file. And i have one doubt that .fna file generated in qiime1 can i use this file in qiime2? Please help me in this doubt. Thanks in advance

Have you checked out the q2-picrust2 plugin ? This will allow you to directly interface with picrust2 using QIIME 2 Artifacts. Thanks! :qiime2: :t_rex:

I think that I have the same question - the code for an .fna file is from the main tutorial on the q2-picrust2 plugin. My codes were: $ qiime vsearch cluster-features-closed-reference \ > --i-table table3.qza \ > --i-sequences rep-seqs3.qza \ > --i-reference-sequences gg_13_5_otu_97.qza \ > --p…

Hey there @Cybele_C ! [image] Cybele_C: Error: Invalid value for “–i-reference-alignment”: File “reference.fna.qza” does not exist. This error means you don't have a file by that name in your current working directory. Do you have a reference alignment in your current working directory?

Hi I think you have not understood the question. The question is how to generate .fna file. I also have same problem.

Hi @faheem - I think you missed my answer above: [image] thermokarst: Have you checked out the q2-picrust2 plugin ? This will allow you to directly interface with picrust2 using QIIME 2 Artifacts. Thanks! :qiime2:

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.fna file in picrust2 in qiime2

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