Finding differential taxa with six groups 6 groups


What would be the appropriate approach to identify statistically differential taxa between 6 groups? I was planning to use ANCOM and perform multiple pairwise comparisons. Thank you for your help.


Hi @Victoria_Ruiz,

That would work — and is probably the best thing to do if you want an ANCOM-style test. Unfortunately, I am not aware of a post-hoc test (e.g., an ANCOM-Tukey test) that is specific to ANCOM that might simplify this process. Check out this forum thread — it sounds like a newer version of ANCOM is available that may support this, and the developers are planning to add that version to QIIME2 in the near future.

You could also check out q2-gneiss. That method is quite useful for working with multivariate datasets, which it sounds like you might be working with, but the downside is that the method can be a bit difficult to interpret if you haven’t worked with it before.

I hope that helps!

1 Like

An off-topic reply has been split into a new topic: Is this sequence depth distribution normal?

Please keep replies on-topic in the future.

This topic was automatically closed 31 days after the last reply. New replies are no longer allowed.