File is not a(n) PairedEndFastqManifestPhred33 file:

[desiree757 (Desiree)]

Hi I using a new set of files to perform Qiime with and I keep getting this error message:

There was a problem importing microbiomegs.csv:

microbiomegs.csv is not a(n) PairedEndFastqManifestPhred33 file:

File referenced on line 2 could not be found (/home/smith5mr/gartersnakemay/Female1-1_S13_L001_R1_001.fastq.gz).

Below is a link to my previous question regarding manifest files, I believe it did not work this time because they are new files. I also have attached my new manifest file. microbiomegs.csv (3.9 KB)

Thank you in advance.

Fastq manifest formats help

me again! I am attempting to do something different with my data as I did not make a manifest file first. I am having difficulty figuring out how to create the.csv as I find the tutorial online hard to understand. Where am I typing the names of these samples into? Do I go to excel to make the manifest ? Also what is each indicative of in the following? sample-id,absolute-filepath,direction # Lines starting with '#' are ignored and can be used to create # "comments" or even "comment out" entries…

[colinbrislawn (Colin J Brislawn)]

Hello again Desiree,

Thanks for posting your full error message. I think this line says it all:

File referenced on line 2 could not be found (/home/smith5mr/gartersnakemay/Female1-1_S13_L001_R1_001.fastq.gz).

So the file isn't in that path... Maybe there's a mispellling in the file path? I know I do that all the time. Keep in mind that the path is case sensitive, so gartersnakemay and GartersnakeMay would be considered different folders and could cause this "could not be found" error.

Let me know what you find!

Colin