Faith_pd visualization in Alpha diversity

I having some problem to generate alpha diversity.
Until this step everything is ok, but when I do this command:
qiime diversity alpha-group-significance
--i-alpha-diversity fiath_pd_vector.qza
--m-metadata-file metadatafile.tsv
--o-visualization faith_pd_vector.qzv

I receive error message:

Plugin error from diversity:

Metadata does not contain any columns that satisfy this visualizer's requirements. Theremust be at least one metadata column that contains categorical data, isn't empty, doesn't consist of unique values, and doesn't consist of exactly one value.

Debug info has been saved to /tmp/qiime2-q2cli-err-5hmp4_c9.log

I have two samples and metadata.tsv is as follows.

I did the validation on 'keemei' in google sheets.

Hi @kyeong_yun ,

It sounds like you don’t have enough data for a comparison. Your columns are either all unique values (#SampleID, Description, and Type) or only consist of one value (BodySite).

You’re going to have a really hard time doing any statistics with just two samples. (We had a big discussion about some of that here, recently.) However, the long and short of it is that you can’t do statitics with just two samples, and that there are no comparisons to be made.



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