Facing problem while importing single end sequences

[Rishikesh12 (Rishikesh Dash)]

I have 24 samples in the fastq.gz format. all reads are single end and i want to import those data into qiime2 but it throwing some errors. I don't have barcode sequences data. Can i proceed without barcode sequences or barcode sequences are necessary for importing.
I am attaching the snap that my sequences are in which format and the error i am getting while importing.

All responses are invited.
Thank you
Rishikesh Dash

[llenzi (Luca)]

Hi @Rishikesh12,

In your situation I would suggest you to import your data via manifest file,
please have a look at the importing tutorial (Importing data — QIIME 2 2021.11.0 documentation) and let us know.

Hope it helps,
Luca

[Rishikesh12 (Rishikesh Dash)]

Thank you so much for your information. I will try this and get back 2 you if i am struck at any part.

Thank you
Rishikesh