Hi,
I just had a quick question about ANCOM analysis through QIIME. I’ve been trying to run ANCOM on my taxonomic data (to see whether there are significant differences between 3 categories of patients: healthy, treated, and untreated/diseased). I used this command:
qiime composition ancom \
–i-table comp-table.qza
–m-metadata-file metadata.tsv
–m-metadata-column subject-type
–o-visualization ancom-subject-type.qzv
–verbose
Although I did get an output, I also got the F_onewayConstantInputWarning error:
/Users/lyskae/opt/anaconda3/envs/qiime2-2020.11/lib/python3.6/site-packages/scipy/stats/stats.py:3641: F_onewayConstantInputWarning: Each of the input arrays is constant;the F statistic is not defined or infinite
warnings.warn(F_onewayConstantInputWarning())
I’m unsure as to how to fix this, and whether this affects the analysis. Any help would be greatly appreciated!