I have two different 16S rRNA gene data sets of the microbiome of a species
i) First dataset is only V4 region (using #515f- TGCCAGCMGCCGCGGTAA and #806r GGACTACNNGGGTATCTAAT primer)
ii). 2nd dataset is V3-V3 region (using standard Illumina primers Forward PrimerTCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG and Reverse Primer = TCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC)
I want to classify only the V4 region from both data sets,
Would it be possible to extract the V4 region form the 2nd data set before denoising reads with DADA2?
Looking forward to hearing from you
Thanks in advanced