extracting the reads silva version 138.1

[thermokarst]

Hi @kedi!

Please check out this response from @SoilRotifer, regarding a very similar error message:

Plugin error from feature-classifier for invalid character

Hi @microbiomeAnalyst, The sequences you download from SILVA are in the form of RNA not DNA, as suggested by the error: Fortunately we have a great plugin which can handle FeatureData[RNASequence] and convert them to DNA (FeatureData[Sequence]) and prepare SILVA data for use as a reference sequence / taxonomy database: https://forum.qiime2.org/t/processing-filtering-and-evaluating-reference-sequence-data-with-rescript/15494 -Take it for a spin! -Mike

Hope that helps!

:qiime2: