I have a couple of questions.
We did full length 16S+18S sequencing. Our data have both 16S and 18S sequences in the same file. I would like to assign taxonomy to this dataset. I am trying to train a classifier for both 16S and 18S combined and would like to know for the step of extracting reads how do I assign more than 1 primer pair?
Would it look something like this:
qiime feature-classifier extract-reads
–p-f-primer primerF1 primerF2
–p-r-primer primerR1 primerR2
On the page: Data resources — QIIME 2 2021.11.0 documentation
Naive Bayes classifiers trained on:
- Silva 138 99% OTUs full-length sequences (MD5:
Is this classifier for 16S+18S, or just 16S?
Pre-formatted SILVA reference sequence and taxonomy files:
Are these files for both 16S and 18S or just 16S?