Hello everyone, I am new to this forum and after spending two days surveying this forum to solve my problem, I am finally asking for help.
I need to mention that I have already went through “Moving Picture” and “Atacama soil microbiome” tutorials and successfully completed them.
Next I am experimenting to do analysis of data downloading from this run: https://www.ncbi.nlm.nih.gov/sra/SRX5598268
run file: https://sra-pub-src-2.s3.amazonaws.com/SRR8809301/HOL_UK2_S389_Prokaryotes_SR1.fastq
I am downloading Original data in fastq file. And I am trying to follow manifest protocol to import the artifact but I am unable to do so.
I just have two questions: 1) Am I doing it correct to follow “Fastq manifest” formats guide and using PairedEndFastqManifestPhred33V2 commands to import my data?
- Is there any way to study the attributes example barcode etc of current run file?
Thank you for your help guys!