Error while using metadata-based filtering

[colinbrislawn (Colin J Brislawn)]

Thank you for the update Kelly,

Just for reference, here is one of the previous conversations about using positive and negative controls.

Filter Controls or Not to Filter Controls

Hello Everyone, I have been trying to determine the best way to filter my stream biofilms eDNA samples. I have negative extraction controls and PCR controls that I included in my library prep. There seems to be a lot of debate over the best way to remove features found in control samples. I agree removing any feature found in a control could be biased but I also don’t feel comfortable ignoring features completely that are found in my controls. Should we be filtering control features that repres…

Colin