Hi Mike, Thanks so much for this tutorial! Learned a lot! Everything went well with this: qiime rescript dereplicate \ --i-sequences trnL-ref-seqs.qza \ --i-taxa trnL-ref-tax.qza \ --p-mode 'uniq' \ --p-threads 8 \ --o-dereplicated-sequences trnL-ref-seqs-derep.qza \ --o-d…

Hi @Birong , It appears you are using a 2-year old version of :qiime2: / RESCRIPt. [image] Birong: 2020.11 RESCRIPt works best with the most recent versions of QIIME 2. I'd recommend running these RESCRIPt commands in the latest release of QIIME 2 as specified at the beginning of this tutori…

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error while running RESCRIPt

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