I have same problem; I had done PICRUST2 analysis before on a group of my data using qiime2-2022.2 version few months a go using virtual box and everything went very well. Now it seems that qiime does not support running analysis using virtual box, so I installed Ubuntu and then Qiime2 2023.7 and picrust2 on my new laptop with 16 GB of RAM, however checking on the terminal show free space around 7GB ...
"(qiime2-2023.7) wsl@Salma:~/picrust2-combined-cecum-feces$ free
total used free shared buff/cache available
Mem: 7975196 560708 139064 3196 7275424 7111140
Swap: 2097152 0 2097152"
Unfortunately I am stuck at the Fragment insertion step when trying to run this command;
qiime fragment-insertion sepp --i-representative-sequences Combined-Cecum-Feces-rep-seqs.qza --i-reference-database picrust2_default_sepp_ref.qza --output-dir sepp_output --p-threads 1 --p-debug --verbose
Plugin error from fragment-insertion:
Command '['run-sepp.sh', '/tmp/qiime2/wsl/data/935d1ae2-7ea5-4f7c-bcf8-9b8d5eaba6fa/data/dna-sequences.fasta', 'q2-fragment-insertion', '-x', '1', '-A', '1000', '-P', '5000', '-a', '/tmp/qiime2/wsl/data/2028ee20-c65b-470e-9cf5-80e2b4e28d0d/data/aligned-dna-sequences.fasta', '-t', '/tmp/qiime2/wsl/data/2028ee20-c65b-470e-9cf5-80e2b4e28d0d/data/tree.nwk', '-r', '/tmp/qiime2/wsl/data/2028ee20-c65b-470e-9cf5-80e2b4e28d0d/data/raxml-info.txt', '-b', '1']' returned non-zero exit status 1.
See above for debug info. (The debug info is very long but I can save it and send it to you if it may help)
, and I keep getting this error Plugin error from fragment-insertion after 30-40 minutes or more:
So this is my question, could this error be related to a bug for the most updated qiime2 version? I tried to downgrade the install qiime but again I could not run this step, and got an error on rep.seqs.qza showing that it is generated on qiime2. 2023.7 version and it was not compatible for this picrust2 fragment insertion step. Please keep me posted. Thank you.