Hi,
I think I have same problem as @SetaPark had. But, I don't think I am understanding the suggested solution.
I am trying to average my samples by group and visualize the group average using heat map.
The heatmap above list all the individual samples
But I would like to represent by group average (like what @SetaPark explained with the drawing above but in heatmap not in taxabarplot).
I have 22 samples and the are grouped by their fermentation periods.
editedmeta.txt (1.7 KB)
I used
qiime feature-table group
--i-table table-no-mitochondria-no-chloroplast.qza
--p-axis sample
--m-metadata-file editedmeta.txt
--m-metadata-column Fermentation
--p-mode median-ceiling
--o-grouped-table grouped-table.qza
to group the samples, then I ran
qiime feature-table heatmap
--i-table grouped-table.qza
--m-sample-metadata-file editedmeta.txt
--m-sample-metadata-column Fermentation
--p-cluster none
--p-color-scheme seismic
--o-visualization heatmap-group.qzv
and I am having the following error message
Plugin error from feature-table:
The following IDs are not present in the metadata: 'Expired', 'Fourteen', 'None', 'Paste', 'Seven', 'Three', 'Twenty_Eight'
Debug info has been saved to /var/folders/kx/p78zx40x3ng39l4wmlc8t2sc0000gn/T/qiime2-q2cli-err-zdhye0rm.log
So I modified my meta data file and ran the same commend line above.
editedmeta_group.txt (698 Bytes)
and now I am having
There was an issue with loading the file editedmeta_group.txt as metadata:
There was an issue with loading the metadata file:
Metadata IDs must be unique. The following IDs are duplicated: 'Expired', 'Fourteen', 'None', 'Paste', 'Seven', 'Three', 'Twenty_Eight'
There may be more errors present in the metadata file. To get a full report, sample/feature metadata files can be validated with Keemei: https://keemei.qiime2.org
Find details on QIIME 2 metadata requirements here: Metadata in QIIME 2 — QIIME 2 2021.4.0 documentation
Please help!
Thank you.