I have one more question.
I was able to evaluate my data before and after trimming, and now I am having trouble running the last step of GNEISS on my filtered data.
I am using the following command:
qiime gneiss balance-taxonomy \
--i-table variance-filtered-table.qza\
--i-tree hierarchy.qza \
--i-taxonomy Classifier_output.qza\
--p-taxa-level 2 \
--p-balance-name 'y0' \
--m-metadata-file mapping.txt \
--m-metadata-column Site \
--o-visualization y0_taxa_summary.qzv --verbose
And I am getting the following error:
Plugin warning from gneiss:
balance-taxonomy is deprecated and will be removed in a future version of this plugin.
/cluster/biocompute/software/qiime2/qiime2-2019-10/envs/qiime2-2019.10/lib/python3.6/site-packages/qiime2/sdk/action.py:236: FutureWarning: This Visualizer is deprecated and will be removed in a future version of this plugin.
Traceback (most recent call last):
File "/cluster/biocompute/software/qiime2/qiime2-2019-10/envs/qiime2-2019.10/lib/python3.6/site-packages/q2cli/commands.py", line 328, in __call__
results = action(**arguments)
File "</cluster/biocompute/software/qiime2/qiime2-2019-10/envs/qiime2-2019.10/lib/python3.6/site-packages/decorator.py:decorator-gen-286>", line 2, in balance_taxonomy
File "/cluster/biocompute/software/qiime2/qiime2-2019-10/envs/qiime2-2019.10/lib/python3.6/site-packages/qiime2/sdk/action.py", line 240, in bound_callable
output_types, provenance)
File "/cluster/biocompute/software/qiime2/qiime2-2019-10/envs/qiime2-2019.10/lib/python3.6/site-packages/qiime2/sdk/action.py", line 445, in _callable_executor_
ret_val = self._callable(output_dir=temp_dir, **view_args)
File "/cluster/biocompute/software/qiime2/qiime2-2019-10/envs/qiime2-2019.10/lib/python3.6/site-packages/q2_gneiss/plot/_plot.py", line 70, in balance_taxonomy
num_features = taxa_df.loc[num_clade.subset()]
File "/cluster/biocompute/software/qiime2/qiime2-2019-10/envs/qiime2-2019.10/lib/python3.6/site-packages/pandas/core/indexing.py", line 1424, in __getitem__
return self._getitem_axis(maybe_callable, axis=axis)
File "/cluster/biocompute/software/qiime2/qiime2-2019-10/envs/qiime2-2019.10/lib/python3.6/site-packages/pandas/core/indexing.py", line 1839, in _getitem_axis
return self._getitem_iterable(key, axis=axis)
File "/cluster/biocompute/software/qiime2/qiime2-2019-10/envs/qiime2-2019.10/lib/python3.6/site-packages/pandas/core/indexing.py", line 1133, in _getitem_iterable
keyarr, indexer = self._get_listlike_indexer(key, axis, raise_missing=False)
File "/cluster/biocompute/software/qiime2/qiime2-2019-10/envs/qiime2-2019.10/lib/python3.6/site-packages/pandas/core/indexing.py", line 1092, in _get_listlike_indexer
keyarr, indexer, o._get_axis_number(axis), raise_missing=raise_missing
File "/cluster/biocompute/software/qiime2/qiime2-2019-10/envs/qiime2-2019.10/lib/python3.6/site-packages/pandas/core/indexing.py", line 1177, in _validate_read_indexer
key=key, axis=self.obj._get_axis_name(axis)
KeyError: "None of [Index(['D_0__Bacteria;D_1__Actinobacteria;D_2__Actinobacteria;D_3__Propionibacteriales;D_4__Nocardioidaceae;D_5__Nocardioides',\n 'D_0__Bacteria;D_1__Firmicutes;D_2__Clostridia;D_3__Clostridiales;D_4__Lachnospiraceae;D_5__Lachnoclostridium 10;D_6__uncultured bacterium',\n 'D_0__Bacteria;D_1__Firmicutes;D_2__Clostridia;D_3__Clostridiales;D_4__Lachnospiraceae;D_5__Lachnospiraceae UCG-006;D_6__uncultured bacterium',\n 'D_0__Bacteria;D_1__Firmicutes;D_2__Clostridia;D_3__Clostridiales;D_4__Lachnospiraceae;D_5__Lachnoclostridium',\n 'D_0__Bacteria;D_1__Bacteroidetes;D_2__Bacteroidia;D_3__Bacteroidales;D_4__Porphyromonadaceae;D_5__Porphyromonas',\n 'D_0__Bacteria;D_1__Firmicutes;D_2__Clostridia;D_3__Clostridiales;D_4__Lachnospiraceae;D_5__uncultured;Ambiguous_taxa',\n 'D_0__Bacteria;D_1__Firmicutes;D_2__Clostridia;D_3__Clostridiales;D_4__Family XI;D_5__Parvimonas',\n 'D_0__Bacteria;D_1__Firmicutes;D_2__Clostridia;D_3__Clostridiales;D_4__Ruminococcaceae;D_5__Ruminococcaceae UCG-007',\n 'D_0__Bacteria;D_1__Bacteroidetes;D_2__Bacteroidia;D_3__Bacteroidales;D_4__Paludibacteraceae;D_5__uncultured;D_6__uncultured Paludibacter sp.',\n 'D_0__Bacteria;D_1__Firmicutes;D_2__Clostridia;D_3__Clostridiales;D_4__Ruminococcaceae;D_5__Ruminiclostridium 9;Ambiguous_taxa',\n ...\n 'D_0__Bacteria;D_1__Proteobacteria;D_2__Alphaproteobacteria;D_3__Rhizobiales;D_4__Rhizobiaceae;D_5__Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium;D_6__uncultured Rhizobium sp.',\n 'D_0__Bacteria;D_1__Proteobacteria;D_2__Gammaproteobacteria;D_3__Betaproteobacteriales;D_4__Hydrogenophilaceae;D_5__Tepidiphilus',\n 'D_0__Bacteria;D_1__Lentisphaerae;D_2__Lentisphaeria;D_3__Victivallales;D_4__vadinBE97;D_5__uncultured rumen bacterium;D_6__uncultured rumen bacterium',\n 'D_0__Bacteria;D_1__Firmicutes;D_2__Bacilli;D_3__Bacillales;D_4__Bacillaceae;D_5__Bacillus;D_6__Bacillus thermoamylovorans',\n 'D_0__Bacteria;D_1__Proteobacteria;D_2__Gammaproteobacteria;D_3__Betaproteobacteriales;D_4__Neisseriaceae;D_5__Neisseria',\n 'D_0__Archaea;D_1__Euryarchaeota;D_2__Methanobacteria;D_3__Methanobacteriales;D_4__Methanobacteriaceae;D_5__Methanobrevibacter',\n 'D_0__Bacteria;D_1__Actinobacteria;D_2__Thermoleophilia;D_3__Solirubrobacterales;D_4__Solirubrobacteraceae;D_5__uncultured',\n 'D_0__Bacteria;D_1__Firmicutes;D_2__Clostridia;D_3__Clostridiales;D_4__Lachnospiraceae;D_5__Anaerostipes',\n 'D_0__Bacteria;D_1__Firmicutes;D_2__Clostridia;D_3__Clostridiales;D_4__Ruminococcaceae;D_5__Oscillibacter;D_6__uncultured bacterium',\n 'D_0__Bacteria;D_1__Patescibacteria;D_2__Saccharimonadia;D_3__Saccharimonadales;D_4__Saccharimonadaceae'],\n dtype='object', name='Feature ID', length=672)] are in the [index]"
Plugin error from gneiss:
"None of [Index(['D_0__Bacteria;D_1__Actinobacteria;D_2__Actinobacteria;D_3__Propionibacteriales;D_4__Nocardioidaceae;D_5__Nocardioides',\n 'D_0__Bacteria;D_1__Firmicutes;D_2__Clostridia;D_3__Clostridiales;D_4__Lachnospiraceae;D_5__Lachnoclostridium 10;D_6__uncultured bacterium',\n 'D_0__Bacteria;D_1__Firmicutes;D_2__Clostridia;D_3__Clostridiales;D_4__Lachnospiraceae;D_5__Lachnospiraceae UCG-006;D_6__uncultured bacterium',\n 'D_0__Bacteria;D_1__Firmicutes;D_2__Clostridia;D_3__Clostridiales;D_4__Lachnospiraceae;D_5__Lachnoclostridium',\n 'D_0__Bacteria;D_1__Bacteroidetes;D_2__Bacteroidia;D_3__Bacteroidales;D_4__Porphyromonadaceae;D_5__Porphyromonas',\n 'D_0__Bacteria;D_1__Firmicutes;D_2__Clostridia;D_3__Clostridiales;D_4__Lachnospiraceae;D_5__uncultured;Ambiguous_taxa',\n 'D_0__Bacteria;D_1__Firmicutes;D_2__Clostridia;D_3__Clostridiales;D_4__Family XI;D_5__Parvimonas',\n 'D_0__Bacteria;D_1__Firmicutes;D_2__Clostridia;D_3__Clostridiales;D_4__Ruminococcaceae;D_5__Ruminococcaceae UCG-007',\n 'D_0__Bacteria;D_1__Bacteroidetes;D_2__Bacteroidia;D_3__Bacteroidales;D_4__Paludibacteraceae;D_5__uncultured;D_6__uncultured Paludibacter sp.',\n 'D_0__Bacteria;D_1__Firmicutes;D_2__Clostridia;D_3__Clostridiales;D_4__Ruminococcaceae;D_5__Ruminiclostridium 9;Ambiguous_taxa',\n ...\n 'D_0__Bacteria;D_1__Proteobacteria;D_2__Alphaproteobacteria;D_3__Rhizobiales;D_4__Rhizobiaceae;D_5__Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium;D_6__uncultured Rhizobium sp.',\n 'D_0__Bacteria;D_1__Proteobacteria;D_2__Gammaproteobacteria;D_3__Betaproteobacteriales;D_4__Hydrogenophilaceae;D_5__Tepidiphilus',\n 'D_0__Bacteria;D_1__Lentisphaerae;D_2__Lentisphaeria;D_3__Victivallales;D_4__vadinBE97;D_5__uncultured rumen bacterium;D_6__uncultured rumen bacterium',\n 'D_0__Bacteria;D_1__Firmicutes;D_2__Bacilli;D_3__Bacillales;D_4__Bacillaceae;D_5__Bacillus;D_6__Bacillus thermoamylovorans',\n 'D_0__Bacteria;D_1__Proteobacteria;D_2__Gammaproteobacteria;D_3__Betaproteobacteriales;D_4__Neisseriaceae;D_5__Neisseria',\n 'D_0__Archaea;D_1__Euryarchaeota;D_2__Methanobacteria;D_3__Methanobacteriales;D_4__Methanobacteriaceae;D_5__Methanobrevibacter',\n 'D_0__Bacteria;D_1__Actinobacteria;D_2__Thermoleophilia;D_3__Solirubrobacterales;D_4__Solirubrobacteraceae;D_5__uncultured',\n 'D_0__Bacteria;D_1__Firmicutes;D_2__Clostridia;D_3__Clostridiales;D_4__Lachnospiraceae;D_5__Anaerostipes',\n 'D_0__Bacteria;D_1__Firmicutes;D_2__Clostridia;D_3__Clostridiales;D_4__Ruminococcaceae;D_5__Oscillibacter;D_6__uncultured bacterium',\n 'D_0__Bacteria;D_1__Patescibacteria;D_2__Saccharimonadia;D_3__Saccharimonadales;D_4__Saccharimonadaceae'],\n dtype='object', name='Feature ID', length=672)] are in the [index]"
See above for debug info.
Any ideas on how to fix this issue? The new file that I havenāt used previously is the one specified under the --i-taxonomy
input command. So this error might has something to do with using the wrong input taxonomy file.
Thanks again, Joao